Detection of Ureaplasma spp. in semen samples from sheep in Brazil
نویسندگان
چکیده
A study was conducted to verify the presence of mycoplasmas and ureaplasmas DNA in sheep semen samples from the State of Pernambuco. The PCR assay was conducted of according with standard protocols with generic primers. Mollicutes DNA was detected in 26.0% and Ureaplasma spp. in 12.0% of semen samples.
منابع مشابه
Molecular characterization of ureaplasmas isolated from reproductive tract of goats and sheep from Brazil
Ovine/caprine ureaplasmas have not yet been assigned a species designation, but they have been classified into nine serotypes. Herein ureaplasmas were searched for in 120 samples of vulvo vaginal mucous from sheep and 98 samples from goats at 17 farms. In addition, semen samples were collected from 11 sheep and 23 goats. The recovered ureaplasma were from sheep and goats from animals without an...
متن کاملشناسایی اوره آ پلاسما اوره آ لیتیکوم در نمونه های کلینیکی مایع منی مردان نابارور با استفاده از روش مولکولی
Background and purpose: Ureaplasma urealyticum is one of the most common causes of Non-Gonococcal Urethritis (NGU). Asymptomatic clinical infection caused by this bacterium can cause malnutrition of the sexual attachment glands and its presence in semen contributes to lower fertility. The aim of this study was to identify Ureaplasma urealyticum in semen of infertile men using PCR method as an a...
متن کاملتعیین ژنوتیپ مولکولی گونه های اوره آپلاسما در زنان مبتلا به عفونت های ژنیتال با روش16S-23S rDNA PCR- RFLP
Introduction & Objective: So far, despite the wide range of methods such as analytic methods used for differentiation of Mycoplasma, the diagnosis of Mycoplasma species is still difficult. Generally the low-level discriminatory power of serological methods because of the rapid changes in size and phase of the dominant antigens in the immune cell surface of Mycoplasmas greatly limits their app...
متن کاملتعیین ژنوتیپ مولکولی گونه های اوره آپلاسما در زنان مبتلا به عفونت های ژنیتال با روش16S-23S rDNA PCR- RFLP
Introduction & Objective: So far, despite the wide range of methods such as analytic methods used for differentiation of Mycoplasma, the diagnosis of Mycoplasma species is still difficult. Generally the low-level discriminatory power of serological methods because of the rapid changes in size and phase of the dominant antigens in the immune cell surface of Mycoplasmas greatly limits their app...
متن کاملMolecular detection of Theileria spp in sheep and vector ticks in Fasa and Kazeroun areas, Fars Province, Iran
A molecular survey was performed for identification Theileria spp in sheep and ticks during from 2010-2011 in Fasa and Kazeroun areas, Fars province, Iran. A total of 100 sheep from different flocks were clinically examined and blood samples with ixodid ticks collected. The prepared blood smears from capillary vein of ear were stained with giemsa methods and examined by using light microscope. ...
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